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Bio-Synthesis offers DyLight™ 550 fluorescent dye oligonucleotide labeling services.  DyLight™ 550 has a bright orange to red fluorescence rhodamine derivative dye.  This dye can be used to replace Alexa555, TRITC and Cy3.

DyLight™ 550 dye can be incorporated at any position within an Oligonucleotide. A primary amine modified oligonucleotide is used to covalently attach with dye via NHS conjugation chemistry; other cross-linking chemistries are also available depending on particular project specifications. Dual HPLC purification follow by additional step of ultrafiltration is used to remove failure sequences during first HPLC purification,  after labeling, the second HPLC is performed to remove unlabeled oligonucleotides and excessive dye in order to obtain a full length labeled oligonucleotide.

Contact us for DyLight 550 fluorescent dye oligonucleotide labeling services.

Product Information

 

Product Name:

DyLight 550 Oligonucleotide Labeling

Category:

Fluorescent dye label, Alexa555, Cy3 and TRITC Alternative Dye

Modification Code:

[DyLight550]

Exact Mass:

1040

Excitation (nm):

562

Emission (nm):

576

Extinction Coefficient, ε(λ):

150,000

Purification:

Dual HPLC

Delivery Format:

Lyophilized

Shipping Conditions:

Room Temperature

Storage Conditions:

-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.